stock solution of 50x TAE buffer: electrophoresis buffer
242g of tris base
57.1 ml of glacial acetic acid
100 ml of 0.5M EDTA (pH 8.0)
TAE yields a better resolution of DNA fragments.
working solution: 1x TAE
40mM Tris acetate
1mM EDTA
(if making from scratch i believe)
stock solution of 50x TAE buffer: electrophoresis buffer
242g of tris base
57.1 ml of glacial acetic acid
100 ml of 0.5M EDTA (pH 8.0)
TAE yields a better resolution of DNA fragments.
working solution: 1x TAE
40mM Tris acetate
1mM EDTA
(if making from scratch i believe)